论文部分内容阅读
目的探讨可溶性促红细胞生成素受体(s EPOR)在肾性贫血(RA)中的临床意义。方法选择136例采用促红细胞生成素(EPO)治疗的RA患者作为RA组,以同期体检中心接受体检的100名健康者作为对照组。RA患者按贫血程度分为重度贫血组[血红蛋白(Hb)<60 g/L]及轻中度贫血组(Hb 60~120 g/L)。用酶联免疫吸附试验(ELISA)分别检测RA组和对照组外周血s EPOR和EPO/s EPOR复合物,用电化学发光法检测外周血甲状旁腺素(PTH),用苦味酸法检测血清肌酐(Cr),并进行统计学分析。结果与对照组比较,RA轻中度贫血组和重度贫血组s EPOR、EPO/s EPOR复合物、PTH、Cr浓度差异有统计学意义(P<0.05)。在重度贫血组s EPOR与Hb有好的相关性(r=-0.650,P<0.05),轻中度贫血组s EPOR与Hb相关性不明显(r=0.135,P<0.05)。结论 RA患者的外周血中存有高于健康对照组的s EPOR,并且可以与外周血中的EPO形成复合物。本研究对于RA机制的进一步研究和临床治疗具有重要意义。
Objective To investigate the clinical significance of soluble erythropoietin receptor (s EPOR) in renal anemia (RA). Methods One hundred and sixty-six RA patients treated with erythropoietin (EPO) were selected as the RA group and 100 healthy subjects undergoing physical examination during the same period as the control group. Patients with RA were divided into severe anemia group [hemoglobin (Hb) <60 g / L] and mild to moderate anemia group (Hb 60 to 120 g / L) according to the degree of anemia. Serum EPOR and EPO / EPOR complex in peripheral blood of RA and control groups were detected by enzyme-linked immunosorbent assay (ELISA). Peripheral blood parathyroid hormone (PTH) was detected by electrochemiluminescence and picric acid Creatinine (Cr), and statistical analysis. Results Compared with the control group, there was significant difference in s EPOR, EPO / EPOR complex, PTH and Cr concentrations between RA mild to moderate anemia group and severe anemia group (P <0.05). There was a good correlation between EPOR and Hb in severe anemia group (r = -0.650, P <0.05). There was no significant correlation between EPOR and Hb in severe anemia group (r = 0.135, P <0.05). Conclusion RA patients have higher levels of s EPOR in the peripheral blood than healthy controls and can form complexes with EPO in the peripheral blood. This study is of great significance for the further study of RA mechanism and clinical treatment.