Evaluation of seedling emergence is a common method used to investigate the vegetation restoration potential of soil seed banks. Previous studies have primarily been conducted in the laboratory using a single water treatment. However, when this method is used, it is dificult to optimize the water conditions for the needs of the individual species of seeds. Additionally, the germination conditions for seedlings in the laboratory are different from those in nature. To avoid these issues, we conducted experiments to activate soil seed banks in the field using different water treatments. The results revealed that: (1) generally, the number of species and quantity of seedlings decrease gradually as the water supply decreases, but that for several species most seedlings did not appear when they were provided with the greatest amount of water, and (2) different species present different germination characteristics. According to the rate of seedling emergence, the species were classified into two germination types: fast and slow. In addition, each species also had a unique climax period of emergence, which was delayed with a decrease in water supply. Therefore, the amount of water provided is vital to seedling emergence, and various water treatments should be evaluated when studying the characteristics of different species in soil seed banks. For the lower reaches of the Tarim River, the optimal soil moisture for seedling emergence ranged from 23% to 28%, and seedling conservation should be strengthened during the climax period of emergence (9-15 days from the day when water became available). These results highlight the importance of the use of an optimal water treatment and demonstrate the danger of using a single treatment method. Our results also emphasize the need for a preliminary study prior to conducting studies to evaluate seed banks. Evaluation of seedling emergence is a common method used to investigate the vegetation restoration potential of soil seed banks. Previous studies have primarily conducted in the laboratory using a single water treatment. However, when this method is used, it is dificult to optimize the water conditions for the needs of the individual species of seeds. To avoid these issues, we conducted experiments to activate soil seed banks in the field using different water treatments. The results revealed that: (1) generally, the number of species and quantity of seedlings decrease gradually as the water supply decreases, but that for several species most seedlings did not appear when they were provided with the greatest amount of water, and (2) different species present different germination characteristics. According to the rate of seedling emergence, the species were classified into In addition, each species also had a unique climax period of emergence, which was delayed with a decrease in water supply. Thus, the amount of water provided is vital to seedling emergence, and various water treatments should be evaluated when studying the characteristics of different species in soil seed banks. For the lower reaches of the Tarim River, the optimal soil moisture for seedling emergence ranged from 23% to 28%, and seedling conservation should be strengthened during the climax period of emergence (9-15 days from the day when water became available). These results highlight the importance of the use of an optimal water treatment and demonstrate the danger of using a single treatment method. Our results also emphasize the need for a preliminary study prior to conducting studies to evaluate seed banks.