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帕金森病(Parkinson′s disease,PD)是一种中枢神经系统慢性进展性疾病.本研究采用双向凝胶电泳(two-dimensional gel electrophoresis,2-DE)分离脑脊液(cerebrospinal fluid,CSF)蛋白,获得2-DE图谱,通过ImageMaster 2D Elite软件分析寻找两组的差异蛋白点.结果显示,PD患者CSF中有4个蛋白点丰度下降,22个蛋白点丰度上升.还利用电喷雾质谱(electrospray ionization-tandem mass spectrometric,ESI-MS)对差异蛋白点进行鉴定,发现丰度上升的蛋白点有电压依赖性钙通道α2/δ1亚基,结合珠蛋白,β2-微球蛋白和阿朴脂蛋白A-IV前体,丰度下降的蛋白点为转铁蛋白和转甲状腺蛋白.研究发现,PD患者与对照组CSF蛋白质表达有明显差异,对差异蛋白进行质谱鉴定并了解它们的功能,为以后进一步研究他们在PD发病机制和病程进展中的作用奠定基础.
Parkinson’s disease (PD) is a chronic progressive disease of the central nervous system.In this study, cerebrospinal fluid (CSF) protein was separated by two-dimensional gel electrophoresis (2-DE) 2-DE patterns were obtained and analyzed by ImageMaster 2D Elite software to find differential protein spots in both groups.The results showed that the abundance of four protein spots in CSF of PD patients decreased and the abundance of 22 protein spots increased.Using electrospray ionization mass spectrometry Electrospray ionization-tandem mass spectrometry (ESI-MS) was used to identify the differentially expressed protein spots. It was found that the abundance of protein spots increased with the voltage-dependent calcium channel α2 / δ1 subunits, haptoglobin, β2-microglobulin and apoliposome Protein A-IV precursor, decreased abundance of protein spots for transferrin and thyroid gland protein.The study found that PD patients and control group, there was a significant difference in CSF protein expression of the differential proteins identified by mass spectrometry and understand their function as After further study of their role in PD pathogenesis and progression of the foundation.