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Nc裸鼠自1984年从日本引入我国,1986年引入中山医科大学称为Nc/s-nu/nu,已广泛应用在肿瘤学,免疫学及药理学等多个领域的研究。小鼠的某些同功酶和异构蛋白具有多态性,已成为研究小鼠遗传结构的标志。本组采用醋酸纤维膜电泳法对21只Nc/s-nu/nu小鼠群体中11个生化标志进行抽样检查,并计算分析其所代表的等位基因在Nc/s-nu/nu小鼠中出现的频率,用Hardy weinherg平衡公式进行吻合度检验,结果发现Nc/s-nu/nu是非近交系小鼠,可作为一个封闭群小鼠,其来源可能与CBA家族小鼠有血缘关系,还可能与RF,RFM,KK等一些品系实验小鼠中某个品系有血缘关系。本实验为我国Nc裸鼠的遗传背景作了初步的研究,也为我校Nc/s-nu/nu小鼠提供了必要的遗传数据。
Nc nude mice were introduced into our country from Japan in 1984. Introduced to Zhongshan Medical University in 1986 as Nc / s-nu / nu, Nc nude mice have been widely used in many fields such as oncology, immunology and pharmacology. Some of the isozymes and isozymes in mice have polymorphisms and have become a hallmark of studying the genetic structure of mice. In this study, 11 biochemical markers of 21 Nc / s-nu / nu mice were sampled by cellulose acetate membrane electrophoresis. The alleles of the 11 alleles in Nc / s-nu / nu mice , We found that Nc / s-nu / nu is a non-inbred mouse, which can be used as a closed group mouse and its origin may be related to the CBA family mice , But also may be related to RF, RFM, KK and some other strains of experimental mice in a lineage. This experiment made a preliminary study on the genetic background of Nc nude mice in our country and provided necessary genetic data for our Nc / s-nu / nu mice.