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通过研究油田微生物驱后油水两相理化性质,发现注入营养剂后采出液水相黏度升高50%以上,油相黏度降低54%,油水两相界面张力降低了24%。通过比较两种细胞破碎方法提取的采出水中细菌总DNA质量,确定使用以超声波细胞破碎法所提取的水样总DNA扩增细菌16SrDNA高可变区V9区,利用变性梯度凝胶电泳(DGGE)法根据DNA片段裂解性质分析不同时期采出水菌群变化。通过对优势条带序列的分析及在GenBank中的比对,发现现场营养剂激活后菌群构成起向简单化,激活后微生物采油常用功能菌假单胞菌属和梭菌为优势菌。说明通过现场营养剂的注入,定向激活了有益功能菌,改善了油藏油水流度比,这些都是油田现场采收率提高的原因。
By studying the physical and chemical properties of oil-water two-phase after oil-field microbial flooding, it was found that the viscosity of oil-water phase increased by more than 50% after injection of nutrient, the viscosity of oil phase decreased by 54% and the interfacial tension decreased by 24%. By comparing the quality of total bacterial DNA extracted from the two kinds of cell disintegration methods, we determined that the 16SrDNA hypervariable region V9 of bacteria was amplified by using the total DNA extracted from the water samples by ultrasonic cell disruption method. The denaturing gradient gel electrophoresis (DGGE ) Method according to the fragmentation of DNA fragments analysis of changes in water production in different periods of flora. Through the analysis of the dominant band sequence and the comparison in GenBank, it was found that the composition of the flora after activation of the nutrient in the field was simplistic, and the dominant functional bacteria Pseudomonas and Clostridium are commonly used for microbial oil recovery after activated. This shows that the beneficial effect of bacteria is enhanced by the injection of on-site nutrient, and the oil-water ratio of reservoirs is improved. These are all the reasons for the increase of field oil recovery.