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目的 观察Behcet病周围血淋巴细胞Fas和FasL的表达及其意义。方法 应用Oligod(T)18和M－MuLv逆反录酶方法,观察19例Behcet病患者和19个正常人周围血淋巴细胞的 Fas和 FasL mRNA表达,并用流动细胞计和免疫荧光法进一步观察T淋巴细胞的Fas和FasL的表达。结果Behcet病组Fas和FasL的表达明显高于对照组。Fas抗原在CD4+和CD8+的表达明显高于对照组,但FasL的表达与对照组无明显差异。结论Behcet病周围血淋巴细胞 Fas和 FasL mRNA的表达明显增高,但其分子表达不平衡,此可能是Behcet病慢性和复发性炎症的一个重要机制。
Objective To observe the expression of Fas and FasL in peripheral blood lymphocytes in patients with Behcet’s disease and its significance. Methods The expressions of Fas and FasL mRNA in peripheral blood lymphocytes of 19 patients with Behcet’s disease and 19 normal individuals were observed by Oligod (T) 18 and M-MuLv reverse transcriptase. Flow cytometry and immunofluorescence were used to observe the expression of T lymphocytes Cell Fas and FasL expression. Results The expression of Fas and FasL in Behcet’s disease group was significantly higher than that in control group. Fas antigen expression in CD4 + and CD8 + was significantly higher than the control group, but FasL expression and control group no significant difference. Conclusions The expression of Fas and FasL mRNA in peripheral blood lymphocytes of Behcet ’s disease is obviously increased, but its molecular expression is not balanced. This may be an important mechanism of chronic and recurrent inflammation in Behcet’ s disease.