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目的:探讨钙蛋白酶10(Calpain10)基因第3内含子SNP19多态性在湖北汉族人群2型糖尿病发生发展中的作用。方法:采用同胞对(家系内对照)和随机病例-时照两种实验设计,结合聚合酶链反应-限制性片断长度多态性(PCR-RFLP)技术分析共403个样本的Calpain10基因第3内含子SNP19多态性,并测定身高、体重、腰围、臀围、血压和空腹血糖等生理指标。结果:两种实验设计中对照组与病例组的基因型和基因频率均无显著差异(p 0．05)。结论:Calpain10基因SNP19多态性在湖北汉族人群2型糖尿病的发生发展中可能不起主要作用。
Objective: To investigate the role of SNP19 polymorphism of the third intron of calpain10 gene in the development of type 2 diabetes in Han nationality in Hubei province. METHODS: Two pairs of experimental designs of sibling pairs (in-house controls) and randomized case-to-case-control samples were used to analyze the expression of Calpain10 gene in 403 samples by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Intron SNP19 polymorphism, and determination of height, weight, waist circumference, hip circumference, blood pressure and fasting blood glucose and other physiological indicators. RESULTS: There was no significant difference in genotype and gene frequency between control and case groups in both experimental designs (p <0.05). Conclusion: SNP19 polymorphism of Calpain10 gene may not play a major role in the occurrence and development of type 2 diabetes in Hubei Han population.