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以薄叶系文心兰Alha′Iwanaga′茎尖诱导形成的原球茎 (PLB)为材料 ,比较不同基本培养基类型 (MS、1/ 2MS、VW )及不同培养方式 (固体培养、液体培养 )对原球茎增殖的影响 ,结果表明 :文心兰PLB增殖以 1/ 2MS为基本培养基配合振荡及回旋培养方式较好 ,固体培养方式因其幼苗形成率高 ,不适合进行文心兰PLB增殖培养
In order to compare the effects of different basic medium types (MS, 1 / 2MS, VW) and different culture methods (solid culture and liquid culture) on the PLBs induced by thin stems of Alha’Iwanaga ’ The results showed that: 1/2 MS medium supplemented with 1/2 MS medium for shaking and cyclotrophic culture was the best method for the proliferation of the cultured native C. sinensis PLB. Because of its high rate of seedling formation, the solid culture method was not suitable for the proliferation of the regenerated to cultivate