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研究了结构相似的吩噻嗪类药物盐酸氯丙嗪(CPZ·HCl)和盐酸异丙嗪(PZ·HCl)与核酸(NA)的相互作用,发现CPZ-NA体系可产生强烈的共振Rayleigh散射(RRS),而PZ-NA反应较微弱.采用量子化学AM1和密度泛函B3LYP方法计算了两个反应的能量关系,讨论了CPZ与DNA分子的结合位点和作用方式,获得一些有意义的结果.RRS法有望成为研究核酸的分子识别和基因药物的分子设计的有用手段.此外,用盐酸氯丙嗪作试剂,RRS法测定核酸具有很高的灵敏度和良好的选择性,因此也为核酸的定量测定提供了一种新的灵敏、简便、快速的新方法.
The interaction between CPZ · HCl and PZ · HCl and nucleic acids (NA) of phenothiazines with similar structure was studied. It was found that the CPZ-NA system could produce intense resonance Rayleigh scattering (RRS), while the PZ-NA reaction is relatively weak.We calculated the energy relationship between the two reactions by AM1 and B3LYP methods and discussed the binding sites and mode of action between CPZ and DNA molecules to obtain some meaningful As a result, the RRS method is expected to be a useful tool to study the molecular recognition of nucleic acids and the molecular design of gene drugs.In addition, with the chlorpromazine hydrochloride as a reagent, the RRS assay has high sensitivity and good selectivity and therefore is also a nucleic acid The quantitative assay provides a new, sensitive, easy and fast new method.