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目的:探讨喉乳头状瘤中上皮生长因子受体(EGFR)和信号转导与转录激活因子3(STAT3)的表达与其生物学行为的关系。方法:采用RT-PCR、免疫组织化学方法和Westernblot,检测42例喉乳头状瘤和15例正常喉黏膜组织中EGFR和STAT3、磷酸化-STAT3(p-STAT3)的mRNA和蛋白质表达情况,同时将蛋白质表达水平与喉乳头状瘤的临床病理参数进行相关性分析。结果:EGFR与STAT3的mRNA在喉乳头状瘤组织表达水平显著高于正常喉组织(P<0.05,P<0.01)。免疫组织化学及Westernblot方法也证实喉乳头状瘤组织中EGFR与STAT3(p-STAT3)的蛋白质表达水平显著高于正常喉组织(P<0.05)。EGFR与STAT3(p-STAT3)蛋白质在喉乳头状瘤组织的表达显著关联(P<0.05),STAT3蛋白与喉乳头状瘤的复发和恶变有明显相关性(P<0.05)。结论:EGFR-STAT3信号转导通路参与喉乳头状瘤的发生,STAT3蛋白的持续活化可能促进喉乳头状瘤的复发与恶变。
Objective: To investigate the relationship between epithelial growth factor receptor (EGFR), signal transducer and activator of transcription 3 (STAT3) expression and biological behavior in laryngeal papilloma. Methods: The mRNA and protein expressions of EGFR, STAT3 and phospho-STAT3 (p-STAT3) in 42 cases of laryngeal papilloma and 15 cases of normal laryngeal mucosa were detected by RT-PCR, immunohistochemistry and Western blot. Correlation analysis between protein expression level and clinical pathological parameters of laryngeal papilloma was made. Results: The mRNA expression of EGFR and STAT3 in laryngeal papilloma was significantly higher than that in normal laryngeal tissue (P <0.05, P <0.01). Immunohistochemistry and Western blot also confirmed that the protein expression of EGFR and STAT3 (p-STAT3) in laryngeal papilloma was significantly higher than that in normal laryngeal tissues (P <0.05). There was a significant correlation between the expression of EGFR and STAT3 (p-STAT3) protein in laryngeal papilloma (P <0.05), and the correlation between STAT3 protein and recurrence and malignant transformation of laryngeal papilloma (P <0.05). Conclusion: The EGFR-STAT3 signal transduction pathway is involved in the pathogenesis of laryngeal papilloma. The sustained activation of STAT3 protein may promote the recurrence and malignant transformation of laryngeal papilloma.