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为了解胃癌组织中多种基因蛋白异常表达及意义，应用免疫组化技术检测５１例手术切除胃癌组织标本中增殖细胞核抗原（ＰＣＮＡ）及Ｐ５３、Ｋ－ｒａｓ、Ｂｃｌ－２蛋白的表达。结果胃癌组织中ＰＣＮＡ标记指数（ＰＣＮＡＬＩ）为５１．２±２０．５，Ｐ５３，Ｋ－ｒａｓ，Ｂｃｌ－２蛋白表达率分别３７．３％，１７．６％和６８．６％，均显著高于正常胃粘膜（Ｐ＜０．０５）。ＰＣＮＡＬＩ与肿瘤大小、淋巴结转移及临床分期密切相关（Ｐ＜０．０５）。肠型胃癌中Ｋ－ｒａｓ、Ｂｃｌ－２蛋白表达率分别为４３．８％和９３．８％，均显著高于胃型胃癌的６．０％（Ｐ＜０．０１），５４．５％（Ｐ＜０．０５），而ＰＣＮＡＬＩ及Ｐ５３蛋白表达率两型胃癌无显著差别。提示ＰＣＮＡ、Ｐ５３、Ｋ－ｒａｓ、Ｂｃｌ－２蛋白异常表达与胃癌的发生发展有关。ＰＣＮＡＬＩ可能对判断胃癌的预后有一定价值。Ｋ－ｒａｓ、Ｂｃｌ－２异常表达可能与肠型胃癌发生有关
To understand the abnormal expression and significance of various gene proteins in gastric cancer, immunohistochemistry was used to detect the expression of proliferating cell nuclear antigen (PCNA) and P53, K-ras and Bcl-2 protein in 51 specimens of surgically resected gastric cancer. Results The PCNA index was 51.2±20.5 in gastric cancer tissues, and the protein expression rates of P53, K-ras and Bcl-2 were 37.3%, 17.6% and 68.6%, respectively. In the normal gastric mucosa (P <0.05). PCNALI was closely related to tumor size, lymph node metastasis, and clinical stage (P<0.05). The expression rates of K-ras and Bcl-2 protein in intestinal type gastric cancer were 43.8% and 93.8%, respectively, which were significantly higher than those in gastric type gastric cancer (6.0%, P<0.01), 54.5%. (P<0.05), but there was no significant difference in the expression rates of PCNALI and P53 protein between the two types of gastric cancer. It is suggested that the abnormal expression of PCNA, P53, K-ras and Bcl-2 proteins is related to the occurrence and development of gastric cancer. PCNALI may have some value in determining the prognosis of gastric cancer. Aberrant expression of K-ras and Bcl-2 may be related to intestinal type gastric cancer