本文阐述了探究性学习、实验操作技能和探究性实验教学模式这三个概念的内涵。同时对高中生物一个重要的验证性实验“探究温度对酶活性的影响”进行了改进,实施了探究性实验教学设计,并进行了教学实践以及分析,旨在探讨如何培养学生的实验操作技能,期望在开展探究性实验教学和培养实验操作技能的道路上做一番探索。 This article elaborates on the connotation of the three concepts of inquiry learning, experimental operating skills, and exploratory experimental teaching models. At the same time, an important verification experiment for senior high school students “impacting the effect of temperature on enzyme activity” was improved. The experimental experimental teaching design was implemented, and teaching practice and analysis were carried out to explore how to cultivate students’ experimental operations. Skills are expected to be explored on the road of conducting exploratory experimental teaching and cultivating experimental operating skills.