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抑霉唑被广泛用来防治由指状青霉菌(Penicillium digitatum)引起的柑橘绿霉病。已有研究表明,柑橘绿霉病菌对抑霉唑的抗性由CYP51基因启动子区5个126-bp转录增强子的简单串联重复和126-bp转录增强子上199-bp的特异性片段插入所引起。基于这2种抗性分子机制,通过设计特异引物和优化条件,建立real-time PCR高通量分子检测技术,用于快速检测柑橘包装贮藏库中绿霉病菌群体对抑霉唑的抗性频率FR,指导科学用药。
Imazalil is widely used to control citrus green mold caused by Penicillium digitatum. Studies have shown that the resistance of citrobacter to imazalil is characterized by a simple tandem repeat of five 126-bp transcription enhancers in the promoter region of the CYP51 gene and a 199-bp specific fragment on the 126-bp transcription enhancer Caused. Based on the two molecular mechanisms of resistance, a real-time PCR high-throughput molecular detection technique was set up by designing specific primers and optimizing the conditions to rapidly detect the frequency of imazalil resistance in the citrus packaging repository FR, to guide scientific medication.