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应用ＮＡＤＰＨ－ｄ组织化学方法研究大鼠胼胝体内一氧化氮合酶（ＮＯＳ）阳性细胞的生后发育。结果发现大鼠出生后１天胼胝体内ＮＯＳ阳性神经元少见，但在侧脑室背侧见有ＮＯＳ阳性细胞层；生后７天已见有ＮＯＳ阳性细胞，但胞体小，其突起呈点线状；生后１４天ＮＯＳ阳性细胞胞体及突起进一步发育，突起呈线状；至２１、２８天时，胼胝体内所见ＮＯＳ阳性细胞与成鼠胼胝体内所见阳性细胞无差异。这些ＮＯＳ阳性细胞散在分布于胼胝体中，有的紧贴于皮质下方并可见有突起伸入皮质；有的位于侧脑室室管膜附近，突起伸入室管膜细胞间，亦见有ＮＯＳ阳性细胞位于室管膜细胞层中；散在于胼胝体中的细胞其突起有的与胼胝体联合纤维并行，有的向各个方向伸展。
NADPH-d histochemical method was used to study the postnatal development of nitric oxide synthase (NOS) positive cells in the corpus callosum of rats. The results showed that NOS positive neurons in the corpus callosum were rare on the 1st day after birth, but NOS positive cells were seen on the dorsal side of the lateral ventricle. NOS positive cells were seen 7 days after birth, but the cell bodies were small, ; 14 days after birth NOS positive cells and further development of cell bodies and protrusions, projections were linear; to 21,28 days, the corpus callosum NOS positive cells and rat corpus callosum see no difference in positive cells. These NOS-positive cells scattered in the corpus callosum, and some close to the cortex and can be seen protruding into the cortex; some in the lateral ventricle ependymal, protruding into the ependymal cells, see also NOS positive cells Located in the ependymal cell layer; scattered in the corpus callosum cells in its protrusion and the corpus callosum fibers in parallel, and some extend in all directions.