论文部分内容阅读
利用菠菜提取物还原制备金纳米颗粒(AuNPs),通过X射线粉末衍射、紫外-可见吸收光谱、扫描电镜、透射电镜等手段对AuNPs进行了表征。生物合成的AuNPs被提取物中生物活性分子包裹,不仅具有良好的生物相容性和水分散性,而且还可以很好地与肿瘤细胞表面的受体蛋白分子结合,使AuNPs深入肿瘤细胞。用红外热成像研究AuNPs光热效应,还进行细胞存活率测试和细胞荧光成像研究。当AuNPs浓度为60μg/mL时,孵化24h后Hela细胞的存活率为91.2%。说明AuNPs具有很好的生物相容性。同样浓度,在近红外光照10min后,Hela细胞的存活率为64.5%。结果表明,金纳米颗粒表现出显著的光热作用,可用于光热杀死肿瘤细胞。
Gold nanoparticles (AuNPs) were prepared by spinach extract reduction. AuNPs were characterized by X-ray powder diffraction, UV-Vis absorption spectra, scanning electron microscopy and transmission electron microscopy. The biosynthetic AuNPs are encapsulated by biologically active molecules in the extract, which not only has good biocompatibility and water dispersibility, but also binds well to the receptor protein molecules on the surface of tumor cells to deeply penetrate AuNPs into the tumor cells. The thermal effects of AuNPs were studied by infrared thermography. Cell viability and cytofluorimetry were also studied. When the concentration of AuNPs was 60μg / mL, the survival rate of Hela cells after 24h incubation was 91.2%. This shows that AuNPs have good biocompatibility. At the same concentration, the survival rate of Hela cells was 64.5% after 10 min of near-infrared light irradiation. The results showed that gold nanoparticles showed a significant photothermal effect, which can be used to kill tumor cells by photothermal.