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目的调查辽宁省农村地区人群Q热立克次体病血清学及虫媒携带病原体状况。方法采集辽宁省14个农村地区人群血液标本,用间接免疫荧光法(indirect immunofluorescence assay,IFA)检测人血清中的Q热立克次体抗体;在辽宁6个地区从羊体表共捕捉蜱71只,实时荧光定量聚合酶链式反应(quantitative polymerase chain reaction method,qPCR)检测蜱虫标本中Q热立克次体DNA。结果血清标本总阳性率为7.33%(男性7.69%,女性6.67%),3个地貌区域的血清阳性率分别为辽东山区10.99%、辽中平原1.15%、辽西山区及草原17.11%,阳性率最高在辽西地区。来自各区的71株蜱未检出Q热立克次体DNA。结论辽宁省内存在人群Q热立克次体感染,流行地主要集中在辽西靠近内蒙地区,多地区Q热传播媒介蜱未检出Q热立克次体。
Objective To investigate the serological and zoonotic pathogen status of Q fever rickettsiosis in rural areas of Liaoning Province. Methods Blood samples were collected from 14 rural areas in Liaoning Province. Antibody against Q hot-rickettsia in human serum was detected by indirect immunofluorescence assay (IFA). Ticks were collected from 6 sheep Only quantitative polymerase chain reaction method (qPCR) was used to detect Q heat Rickettsia DNA in tick specimens. Results The positive rate of serum samples was 7.33% (7.69% for males and 6.67% for females). The seroprevalence of the three geomorphic regions was 10.99% in Liaodong Mountain Area, 1.15% in Liaozhong Plain and 17.11% in Liaoxi Mountainous Area and grassland, respectively. The positive rate was the highest In western Liaoning. 71 ticks from each area did not detect Q hot rickettsial DNA. Conclusions In Liaoning Province, there is a population of Rickettsia gondii infection in the population. The prevalence is mainly concentrated in the western Inner Mongolia area in Liaoning Province, and no Q fever rickettsia was detected in Q heat-transmitted vector ticks in various areas.