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检测代偿期和失代偿期肝硬化患者血及腹水中部分促炎细胞因子 ,从炎症的角度分析肝硬化失代偿发生的可能机理 ,以期利于肝硬化失代偿期患者的临床治疗。一、材料与方法1.材料 :ＩＬ 8、ＩＬ 12、ＴＮＦ αＥＬＩＳＡ法检测试剂盒购于深圳晶美生物试剂公司。细胞总ＲＮＡ提取试
To detect the partial proinflammatory cytokines in blood and ascites of patients with decompensated and decompensated cirrhosis and to analyze the possible mechanism of cirrhosis decompensation from the perspective of inflammation so as to benefit the clinical treatment of patients with decompensated cirrhosis. First, the material and methods 1 material: IL 8, IL 12, TNF α ELISA assay kit was purchased in Shenzhen Crystal US biological reagents company. Cell total RNA extraction test