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目的研究键合多西紫杉醇(TSD)的聚乳酸-聚乙二醇嵌段共聚物(PLA-PEG/TSD)对人前列腺癌细胞PC-3的增殖抑制作用。方法 TSD、PLA-PEG/TSD和聚乳酸-聚乙二醇(PLA-PEG)作用于PC-3细胞,应用MTT法检测各组药物在不同浓度下作用24、48、72 h后PC-3细胞的抑制率,应用流式细胞仪检测PC-3细胞周期变化,应用电镜观察PC-3细胞凋亡的形态学特征。结果 PLA-PEG/TSD和TSD比较,对PC-3细胞的增殖抑制作用相似(P>0.05),并且两组均具有浓度和时间依赖性,随药物浓度和作用时间的增加,抑制率逐渐增高。PLA-PEG和对照组比较,对PC-3细胞的增殖没有影响(P>0.05)。流式细胞仪检测结果显示:PLA-PEG/TSD和TSD两组PC-3细胞明显阻滞于G2/M期,两组相比较差异无统计学意义(P>0.05),与对照组比较差异有统计学意义(P<0.05)。电镜观察:PLA-PEG/TSD和TSD两组PC-3细胞凋亡的形态学特征一致。结论 PLA-PEG/TSD与TSD相比较,抑制PC-3细胞增殖的特点和机制相似,PLA-PEG/TSD在治疗前列腺癌方面有研究价值。
Objective To study the inhibitory effect of PLA-PEG / TSD conjugated with docetaxel on the proliferation of human prostate cancer cell line PC-3. Methods The PC-3 cells were treated with TSD, PLA-PEG / TSD and PLA-PEG. The effects of different concentrations of TSD, PLA-PEG and PLA- The inhibition rate of PC-3 cells was determined by flow cytometry. The morphological characteristics of PC-3 cells were observed by electron microscopy. Results Compared with TSD, PLA-PEG / TSD and TSD showed similar inhibitory effects on the proliferation of PC-3 cells (P> 0.05), and the concentration and time-dependent manner of PLA-PEG / . Compared with the control group, PLA-PEG had no effect on the proliferation of PC-3 cells (P> 0.05). Flow cytometry results showed that PC-3 cells in PLA-PEG / TSD and TSD groups were significantly arrested in G2 / M phase, the difference was not statistically significant (P> 0.05), compared with the control group There was statistical significance (P <0.05). Electron microscopy showed that the morphological characteristics of PC-3 cells in PLA-PEG / TSD and TSD groups were the same. CONCLUSION PLA-PEG / TSD has similarities and differences in the mechanism of inhibiting the proliferation of PC-3 cells compared with TSD. PLA-PEG / TSD is valuable in the treatment of prostate cancer.