论文部分内容阅读
建立了高效液相色谱-串联质谱法同时测定豆芽中6-苄基腺嘌呤、4-氯苯氧乙酸、2,4-D、赤霉素、1-萘乙酸、2-萘氧乙酸、吲哚乙酸、噻苯隆等8种生长调节剂残留量的方法。样品用含1%甲酸甲醇溶液提取,HLB固相萃取柱净化后,经C18色谱柱分离,乙腈-10 mmol/L乙酸铵(含0.1%甲酸)流动相梯度洗脱,串联三重四级杆质谱仪电喷雾负离子多反应监测(MRM)模式下进行检测。结果表明,8种生长调节剂在1~1000μg/L范围内线性关系良好;方法定量限(S/N=10)为0.03~50μg/kg;添加水平为5、10、20μg/kg(萘乙酸和吲哚乙酸为50、100、200μg/kg)时,平均回收率在71.5%~92.3%之间;相对标准偏差(RSD,n=6)为4.6%~13.5%。方法简单快速,灵敏度高,成功应用于市场上绿豆芽和黄豆芽的检测。
A method for simultaneous determination of 6-benzyladenine, 4-chlorophenoxyacetic acid, 2,4-D, gibberellin, 1-naphthaleneacetic acid, 2-naphthyoxyacetic acid and indole in bean sprouts was established by high performance liquid chromatography-tandem mass spectrometry Diallyl acetic acid, thidiazuron and other 8 kinds of growth regulator residual method. The samples were extracted with 1% formic acid in methanol and purified by HLB solid phase extraction column. The residue was separated on a C18 column and eluted with 10 mmol / L ammonium acetate (containing 0.1% formic acid) in acetonitrile. The tandem triple quadrupole mass spectrometry Electrospray Negative Ion Multiple Reaction Monitoring (MRM) mode. The results showed that there was a good linear relationship among 8 kinds of growth regulators in the range of 1 ~ 1000μg / L; the limit of quantification (S / N = 10) was 0.03 ~ 50μg / kg; And indole acetic acid 50,100,200μg / kg), the average recoveries ranged from 71.5% to 92.3%. The relative standard deviations (RSDs, n = 6) ranged from 4.6% to 13.5%. The method is simple, rapid and sensitive, and has been successfully applied to the detection of green bean sprouts and yellow bean sprouts on the market.