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栽培稻种间杂种的高度不育是从非洲栽培稻向亚洲栽培稻转移有利基因的最主要障碍.用携带非洲栽培稻的不育基因S1,以亚洲栽培稻为背景的桥梁亲本与非洲栽培稻杂交,可望大大缓解种间杂种的不育.以亚洲栽培稻合系39和非洲栽培稻IRGC104049为材料,培育出一批BC2F10中间材料,其表现型与轮回亲本表现型一致,供体遗传背景占14.18%.用其与合系39回交培育出的BC3F1花粉和小穗均为半不育,BC3F2群体的花粉育性和小穗育性呈双峰分布,分成半不育和可育两类.第六染色体的RM589、RM588、RM190、RM7639、RM586标记与目标性状相关极显著,并把控制育性的位点定位在RM190和RM7639间,距离分别为3.2cM、4.8cM,说明非洲栽培稻的不育基因S1已导入合系39,紧密连锁的SSR标记RM190和RM7639可用于分子标记辅助选择,S1在合系39背景下的配子消除作用达到79.61%,遗传基本符合单位点孢子体-配子体互作模式.
The most important obstacle to the transfer of favorable gene from African cultivated rice to Asian cultivated rice is high sterility in cultivated rice interspecific hybrids.It is suggested that the male sterility gene S1 carrying African cultivated rice and the bridging parent with Asian cultivated rice as the background and African cultivated rice Is expected to greatly alleviate the interspecific hybrid sterility.A batch of BC2F10 intermediate material was cultivated from the Asian cultivar Yanyi 39 and the African cultivated rice IRGC104049 and the phenotypes of the BC2F10 intermediate phenotype were consistent with the genetic background of the donor Accounting for 14.18% .The pollen and spikelets of BC3F1, which were backcrossed with the line 39, were all semi-sterile, and the pollen fertility and spikelet fertility of the BC3F2 population were bimodal and divided into semi-sterile and fertile two The markers RM589, RM588, RM190, RM7639 and RM586 on chromosome 6 were significantly correlated with the target traits and the loci controlling fertility were located between RM190 and RM7639 at distances of 3.2 cM and 4.8 cM, respectively, indicating that African cultivation Inbred line S1 of rice was introduced into lineage 39. The closely linked SSR markers RM190 and RM7639 could be used for molecular marker-assisted selection. S1 had 79.61% of gametogenic effects in the lineage 39 and inherited well with the unit point sporophyte- Gametophytes each other Mode.