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对以籼稻品种9311为受体、粳稻品种日本晴为供体的3个控制穗颈长度性状的高代回交置换系(C031、C108和C115)进行了农艺性状测定和遗传基础分析。结果表明,除穗颈长度外,3个置换系的株高与9311之间也存在显著或极显著差异,置换系C031的每穗总粒数和千粒重与9311差异显著。通过遗传背景检测,发现置换系C031和C115均含有2个日本晴置换片段,置换系C108含有3个日本晴置换片段。遗传分析表明,3个F2分离群体中穗颈长度的分离均由单个孟德尔因子控制,其加性效应分别为3.09、3.05和–2.04。连锁分析表明,C031和C108与携带置换片段上的分子标记均不连锁,C115与第12号染色体的分子标记存在不同程度的连锁,表明控制C115穗颈长度表型的基因位于第12号染色体上,将其命名为q PNL-12。研究结果为精细定位和克隆该基因奠定了基础。
Three agronomic traits and genetic basis analysis were conducted on three elite reciprocal crosses (C031, C108 and C115) with indica variety 9311 as acceptor and japonica variety Nipponbare as donor. The results showed that there was significant or extremely significant difference between the plant height and 9311 except for the length of panicle neck. The total grain number per panicle and thousand kernel weight per panicle of substitution line C031 were significantly different from that of 9311. Through the genetic background test, we found that C031 and C115 both contain two Nipponbare translocations and the substitutional C108 contains three Nipponbare transplants. Genetic analysis showed that the separation of panicle neck length in three F2 segregation populations was controlled by a single Mendelian factor with additive effects of 3.09, 3.05 and -2.04, respectively. Linkage analysis showed that C031 and C108 were not linked to the molecular markers carrying the substitution fragment, and the molecular markers of C115 and chromosome 12 were linked in varying degrees, indicating that the gene controlling the C115 panicle length phenotype is located on chromosome 12 , Named it PNL-12. The results laid the foundation for fine mapping and cloning of this gene.