论文部分内容阅读
作者用放射免疫测定方法检查了67种市售的丙种球蛋白样品中的乙型肝炎表面抗原(HBsAg)和抗体(Anti-HBs),以确定HBsAg是否污染了丙种球蛋白。检查对象包括用于静脉及肌肉注射的丙种球蛋白制品。每种样品检查3次,蛋白质浓度调至7.5～8％,即HBsAg和Anti-HBs测定方法的最适宜的蛋白质浓度。凡HBsAg或Anti-HBs阳性样品,再用中和试验以确定结果。结果HBsAg阳性11种(16.40％);Anti-HBs阳性48种(71.6％)。现某些市售的丙种球蛋白制品可能含有HBsAg。并且当丙种球蛋白制品所含的免疫复合物中有过量的
The authors examined hepatitis B surface antigen (HBsAg) and antibodies (Anti-HBs) in 67 commercially available gamma globulin samples by radioimmunoassay to determine if HBsAg contaminated gamma globulin. Subjects to be tested included gamma globulin preparations for intravenous and intramuscular injection. Each sample was examined three times and the protein concentration was adjusted to 7.5-8%, ie the optimum protein concentration for the HBsAg and Anti-HBs assays. Where HBsAg or Anti-HBs positive samples, and then neutralization test to determine the results. Results HBsAg was positive in 11 species (16.40%) and anti-HBs was positive in 48 species (71.6%). Certain commercially available gamma globulin preparations may contain HBsAg. And when there is excess in the immune complexes contained in the gamma globulin product