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目的:确定纯化江南卷柏总黄酮的大孔树脂及其工艺参数。方法:以总黄酮的含量为指标,从3种不同型号的大孔树脂中筛选纯化江南卷柏总黄酮的最佳树脂;以江南卷柏总黄酮的回收率为指标,通过单因素试验确定该树脂分离纯化的工艺条件。结果:D101型树脂对江南卷柏总黄酮有较好的吸附和解吸附性能;其纯化江南卷柏总黄酮的较佳工艺条件为:药材量与树脂量的比例为1∶3,以8BV(8倍体积)70%乙醇洗脱,流速为2BV·h-1,树脂每次可重复使用3次。在所确定的工艺条件下,江南卷柏总黄酮纯度可达50%以上,回收率可达85%以上。结论:该工艺条件可用于江南卷柏总黄酮的纯化。
Objective: To determine the purification of total flavonoids of Chamaecrona saponins macroporous resin and its process parameters. Methods: According to the content of total flavonoids, three kinds of macroporous resins were selected as the best resin to purify the total flavonoids of Selaginellata. The recoveries of the total flavonoids from Selaginella nigra were determined by single factor test Resin separation and purification process conditions. Results: The resin D101 had good adsorption and desorption properties to the total flavonoids of Selaginellata var. Pubescens. The optimal conditions for the purification of the total flavonoids from Selaginella concinna were as follows: the ratio of the medicinal material to the resin was 1: 3, Volume) 70% ethanol, the flow rate of 2BV · h-1, the resin can be reused three times each time. Under the determined process conditions, the purity of total flavonoids of Selaginella dahurica can reach more than 50%, and the recovery rate can reach more than 85%. Conclusion: The process conditions can be used for the purification of total flavonoids from Selaginella dahurica.