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背景:神经干细胞为神经系统损伤的修复开辟了新的途径,也是发现某些细胞因子和其作用机制的良好模型,神经干细胞的体外培养、增殖和诱导分化的探讨是这些研究的重要基础。目的:分离培养和鉴定人脑神经干细胞。设计和方法:采用无血清培养基分离和培养人胚胎脑皮质分离和培养神经干细胞,并应用免疫荧光技术进行细胞自我更新能力、巢蛋白表达和多向分化潜能的鉴定。地点和材料:实验在福建医科大学分子医学研究中心实施。实验材料为自然流产的孕龄6～8周的人胚胎脑组织。主要观察指标:人脑神经干细胞在体外培养条件中的增殖、自我更新能力、巢蛋白表达和多向分化潜能。结果:人胚胎脑组织分离培养的细胞具有神经干细胞的特征,并可在体外大量增殖,经诱导可分化为神经元、星形胶质细胞和少突胶质细胞。结论:从人胚胎脑皮质成功分离培养出的神经干细胞,是研究神经干细胞诱导分化的良好模型。
BACKGROUND: Neural stem cells open up new avenues for the repair of nervous system injury and are good models for the discovery of certain cytokines and their mechanisms of action. The in vitro culture, proliferation and differentiation of neural stem cells are important bases for these studies. Objective: To isolate, culture and identify human neural stem cells. DESIGN AND METHODS: Neural stem cells were isolated and cultured in serum-free medium from human embryonic cortex. Immunofluorescence was used to identify the ability of cells to self-renew, nestin expression and multidirectional differentiation potential. Venue and material: The experiment was carried out in Molecular Medical Research Center of Fujian Medical University. Experimental material for spontaneous abortion gestational age 6-8 weeks of human embryonic brain tissue. MAIN OUTCOME MEASURES: Proliferation, self-renewal capacity, nestin expression and multipotential differentiation potential of human neural stem cells in vitro. Results: The cells isolated from human embryonic brain tissue were characterized by neural stem cells and proliferated in vitro. The cells were differentiated into neurons, astrocytes and oligodendrocytes. Conclusion: The successful isolation and culture of neural stem cells from human embryonic cortex is a good model to study neural stem cell differentiation.