论文部分内容阅读
市售油酸是多相脂质体139的主要成分。经 GC—MS 证明,市售油酸是脂肪酸的混合物,油酸(18:1,9C)含量最高,约为70%,用气相色谱法测定多相脂质体139的油酸或市售油酸的含量,样品需预处理。预处理分三步:1.加热使水与油分层。2.用0.5NNaOH—甲醇将分出的油层皂化。3.将脂肪酸的皂化物用甲醇及 BF_3—乙醚酯化。而后进行气相色谱分析,定量精密度一般 CV<3%。
Commercially available oleic acid is the major component of multiphase liposomes 139. GC-MS proved that the commercial oleic acid is a mixture of fatty acids, with the highest content of oleic acid (18:1, 9C), about 70%. Determination of oleic acid or commercial oils of multiphase liposomes 139 by gas chromatography. The acid content of the sample needs to be pretreated. The pretreatment is divided into three steps: 1. Heating delaminates water and oil. 2. Saponify the separated oil layer with 0.5N NaOH-methanol. 3. The saponification of the fatty acid was esterified with methanol and BF3-Ether. After gas chromatography analysis, the quantitative precision is generally CV < 3%.