非蛋白氮之微量测定法原理:燃烧加有硫酸未知液的试管,嗣后加奈斯勒(Nessler)氏显色剂随与燃烧所得的硫酸铵化合获得黄色色度。与同样施以奈斯勒氏显色剂的一定量标准液(即其中含有一定量的氮)比色。试剂:(1)浓硫酸。为了避免氮的侵入需将硫酸保存于特殊的磨口瓶内,另外尚须一个磨口玻璃罩。若 The principle of the non-protein nitrogen micro-assay method: burning a test tube with an unknown solution of sulfuric acid, followed by a Nessler’s color reagent, combined with ammonium sulfate obtained from the combustion to obtain yellow color. A certain amount of standard solution (i.e., a certain amount of nitrogen contained therein) is colorimetrically administered also with a Nessler’s reagent. Reagents: (1) concentrated sulfuric acid. In order to avoid the intrusion of nitrogen, it is necessary to store the sulfuric acid in a special grinding jar, and a grinding glass cover is still required. If