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本文介绍采用自制实验装置和国产仪器建立并改进了连续淋浴式血浴器官生物测定法。本方法具有下列优点:无须将血中被测定的活性物质进行提取、可直接连续地观察体内活性物质含量变化,灵敏度较高、特异性强。选择大鼠胃底、结肠和鸡直肠三种平滑肌制备组成一组生物测定器官,引出猫肾或脾静脉血瀑布式淋浴其上,直接测定血中血管紧张素Ⅱ、前列腺素和儿茶酚胺含量的动态变化。在特异性、灵敏度、梯度和重复性方面均获得较满意的结果。
This article describes the use of self-made experimental equipment and domestic equipment to establish and improve continuous shower blood organ bioassay. The method has the following advantages: no need to extract the active substance in the blood, the content of the active substance in the body can be observed continuously and continuously, the sensitivity is high and the specificity is high. Three kinds of smooth muscle of the stomach, colon, and rectum of rats were selected to make up a group of bioassay organs. Cats kidney or splenic vein was taken out for cascade shower. The dynamic changes of blood angiotensin Ⅱ, prostaglandin and catecholamine were determined directly Variety. Satisfactory results in terms of specificity, sensitivity, gradient and reproducibility.