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本文利用选择性肾动脉注射的方法和离体肾灌注（ｉｓｏｈｔｅｄｐｅｒｆｕｓｅｄｒａｔｋｉｄｎｅｙ，ＩＰＫ）技术，观察了内皮细胞衍生松弛因子（ＥＤＲＦ）的抑制剂Ｎ－ｎｉｔｒｏ－Ｌ－ｉｒｇｉｎｉｎｅ（Ｌ－ＮＡ），抑制肾组织局部产生ＥＤＲＦ后对正常大鼠肾功能的影响。结果表明，（１）选择性肾动脉注射Ｌ－ＮＡ后，大鼠血压无显著变化．肾血流量仅呈下降趋势，但尿量和尿钠排泄量明显减少（４．Ｏ±０．７ｖｓ５．１±０．８μｍｏｌ／ｍｉｎ，Ｐ＜０．０５）；乙酰胆碱只能部分逆转其作用；Ｌ－ＮＡ则能减少乙酰胆碱引起的肾血流量增加。（２）Ｌ－ＮＡ能够直接降低离体肾灌注的尿量和尿钠的排泄。此外，高浓度的Ｌ－ＮＡ还可增加ＩＰＫ的灌注阻力。上述结果有利于解释肾内ＥＤＲＦ在调节肾功能中的作用。
In this paper, selective renal artery injection and isolated renal perfusion (IPK) were used to observe the effects of N-nitro-L-irinine (L-NA), an inhibitor of endothelial cell-derived relaxing factor (EDRF) Effect of local production of EDRF on renal function in normal rats. The results showed that: (1) After selective renal artery injection of L-NA, no significant changes in blood pressure in rats. Renal blood flow only showed a downward trend, but urine output and urinary sodium excretion were significantly decreased (4.O ± 0.7 vs5.1 ± 0.8μmol / min, P <0.05); acetylcholine only partially reversed its effect; L-NA can reduce the increase in renal blood flow caused by acetylcholine. (2) L-NA can directly reduce the excretion of urine and urinary sodium in ex vivo renal perfusion. In addition, high concentrations of L-NA may also increase IPK perfusion resistance. The above results help explain the role of intrarenal EDRF in regulating renal function.