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目的：探索从甲醛固定组织中提取并扩增DNA的方法。方法：采用高pH缓冲液，结合有机溶剂法抽提DNA，选择扩增片段较短的DQa位点作为检测位点。结果：甲醛固定组织中的DNA降解程度随浸泡时间的延长而加重，但浸泡1、5、10个月的3份心脏组织均获得了240bp的扩增产物。
Objective: To explore the method of DNA extraction and amplification from formalin-fixed tissues. Methods: DNA was extracted with high pH buffer and organic solvent method, and the shorter DQa fragment was selected as the detection site. Results: The degree of DNA degradation in formalin-fixed tissues was aggravated with the prolongation of soaking time. However, all the 3 cardiac tissues at 1, 5 and 10 months of soaking obtained a 240 bp amplification product.