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目的从卵巢癌患者的肿瘤组织中提取原代肿瘤细胞,建立条件重编程细胞(conditionally reprogrammed cells,CRCs)长期培养体系,并进行相关药敏试验。方法采用胰酶消化法将卵巢肿瘤组织分散成单个细胞后,用普通完全培养基、条件性培养基、条件性培养基及3T3饲养细胞分别培养,观察其生长情况;并对低代数(P2~P3)的肿瘤细胞进行常用化疗药物的药敏试验。结果在条件性培养基及3T3饲养细胞的作用下,原代卵巢肿瘤细胞可以持续增殖,并保持较快增殖速度。药敏试验结果显示,所培养的原代卵巢肿瘤细胞均对顺铂、紫杉醇、多西他赛敏感,与临床结果一致;雷公藤甲素对卵巢癌细胞有明显抑制作用。结论条件重编程(conditionally reprogramming,CR)技术可以作为一种体外快速有效培养原代卵巢肿瘤细胞的方法,将其作为药敏试验模型在肿瘤化疗及耐药机制的研究中具有重要意义。
Objective To extract primary tumor cells from ovarian cancer patients and establish long-term culture system of conditioned reprogrammed cells (CRCs). Methods The ovarian tumor tissue was dispersed into single cells by trypsin digestion and then cultured with ordinary complete medium, conditioned medium, conditioned medium and 3T3 feeder cells respectively to observe the growth of the ovarian tumor. The effects of low algebra (P2 ~ P3) of the tumor cells commonly used chemotherapy drug susceptibility testing. Results Under the condition of conditioned medium and 3T3 feeder cells, the primary ovarian tumor cells could proliferate continuously and maintain a faster proliferation rate. Susceptibility test results showed that the primary ovarian tumor cells were sensitive to cisplatin, paclitaxel, docetaxel, and clinical results; Triptolide on ovarian cancer cells were significantly inhibited. CONCLUSIONS: Conditionally reprogramming (CR) can be used as a method to rapidly and effectively culture primary ovarian tumor cells in vitro. It is of great significance as a drug susceptibility test model in the study of chemotherapy and drug resistance mechanisms of tumors.