目的：确定杜氏利什曼原虫39KD抗原基因的基因分析与同源性。方法：以杜氏利什曼原虫39KD抗原基因为探针，与不同种株的利什曼原虫进行Southem杂交，确定不同种株的利什曼原虫该基因的变化，并将该基因序列测定后，进行DNA序列数据库检索，分析其同源性，并进行数据库登记。结果：杜氏利什曼原虫不同地域株及婴儿利什曼原虫基因组中均含有该基因，并且无明显定位变化，整个基因在DNA数据库中无完全DNA同源序列，在GenBanK中的序列号为3280。结论：该抗原基因是利什曼原虫保守基因。 OBJECTIVE: To determine the genetic analysis and homology of the 39KD antigen of Leishmania donovani. Methods: The Leishmania donovani 39KD antigen gene was used as a probe to detect Leishmania spp. With different species of Leishmania spp. The variation of Leishmania spp. In different strains was determined. After sequencing of the gene, DNA sequence database search, analysis of its homology, and database registration. Results: The gene was located in the genomes of Leishmania donovani and Leishmania infantum, and there was no obvious change in its location. The whole gene had no complete DNA homology in the DNA database. The GenBank accession number was 3280 . Conclusion: This antigen gene is a Leishmania conserved gene.