论文部分内容阅读
目的 选用前列腺素合成抑制剂布洛芬（Ｉｂｕ）对创伤后细胞免疫功能变化的作用。方法 Ｂａｌｂ／ｃ小鼠行创伤性截肢手术后分Ｉｂｕ 组（腹腔注射Ｉｂｕ １ｍ ｇ／ｋｇ，ｎ＝ ２８）和生理盐水组（腹腔注射生理盐水０．５ｍ ｌ，ｎ＝ ２８），正常小鼠作为对照组。于术后１、３、５、７ 天分别每组取７ 只小鼠测其淋巴细胞增殖功能和自然杀伤细胞（ＮＫ 细胞）活性。结果 创伤后小鼠脾脏淋巴细胞增殖功能明显下降（Ｐ＜ ０．０１），于第７ 天尚未恢复（Ｐ＜ ０．０５）；Ｉｂｕ 组小鼠在创伤后也有明显抑制（第１ 天Ｐ＜ ０．０１，第３ 天Ｐ＜ ０．０５），第５天开始恢复（Ｐ＞ ０．０５），而与生理盐水组比，创伤后第１ 天即呈显著增高（Ｐ＜ ０．０１）。创伤后给Ｉｂｕ 小鼠的的ＮＫ 细胞杀伤活性也有提高，虽较对照组明显降低（Ｐ＜ ０．０１），但第１、３ 天均较生理盐水组明显提高（Ｐ＜ ０．０５ 或０．０１）。结论 布洛芬可部分提高创伤后小鼠受抑制的细胞免疫功能。
Objective To investigate the effect of prostaglandin synthesis inhibitor ibu on cellular immune function after trauma. Methods Balb / c mice were randomly divided into 4 groups: Ibu group (1μg / kg ip, n = 28) and normal saline group (0.5ml saline group, n = 28) As a control group. On the 1st, 3rd, 5th and 7th day postoperatively, 7 mice in each group were measured for lymphocyte proliferation and natural killer (NK) cell activity. Results The proliferation of spleen lymphocytes in trauma mice was significantly decreased (P <0.01), but not recovered on the 7th day (P <0.05). The mice in Ibu group were also significantly inhibited after trauma (P < (P <0.01, P <0.05 on the 3rd day), recovered on the 5th day (P> 0.05), but significantly increased on the 1st day after trauma compared with the saline group (P <0.01) . Compared with the control group, the killing activity of NK cells in Ibu mice after trauma was also significantly increased (P <0.01 or P <0.01) .01). Conclusion Ibuprofen can partially improve the cellular immune function suppressed in traumatic mice.