目的　为研究治疗白血病的有效药物。方法　将槲皮素作用于培养的 NB4细胞株 ,应用 RT- PCR及western blot方法 ,研究不同浓度槲皮素 (30、6 0、90μm)诱导细胞 2 4、48、72、96、12 0 h后 ,白血病 NB4细胞株中突变型 p5 3基因及蛋白表达的情况。结果　证实其 30～ 90 μm浓度时对突变型 p5 3基因没有影响 ,而对突变型 p5 3蛋白的表达产生抑制作用。结论　为槲皮素应用于白血病的研究提供了有效手段 The purpose is to study the effective treatment of leukemia drugs. Methods Quercetin was applied to cultured NB4 cells. RT-PCR and western blotting were used to investigate the effects of quercetin (30, 60, 90μm) on the proliferation of NB4 cells at 24, 48, 72, 96 and 120 h , The leukemia NB4 cell line mutation of p5 3 gene and protein expression. The results confirmed that the 30-90 μm concentration had no effect on the mutant p5 3 gene but inhibited the expression of the mutant p5 3 protein. Conclusions for quercetin applied to the study of leukemia provides an effective means