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用垂直板聚丙烯酰胺凝胶电泳方法对萝卜与芥菜属间杂种F1(RSjF1-1)的叶片酯酶同工酶的酶谱与相同时期的亲本的酯酶同工酶的酶谱比较,既有来自母本的酶带,也有来自父本的酶带,是双亲的互补型。另外还有2条亲本所没有的酶带。 RSjF1-1的花蕾酯酶同工酶的酶谱与相同时期的亲本的酯酶同工酶酶谱比较,结果与对叶片的酶谱分析结果相符合,仍然是双亲的互补型,并且有2条亲本所没有的酶带。在RSjF1-1的叶片和花蕾的酯酶同工酶酶谱中所出现的几条双亲所没有的酶带,活性都较强或很强,多次重复的结果表明是新的酶带。证明RSjF1-1是一种新的遗传类型。确系为萝卜与芥菜的属间杂种。
Comparison of the zymogram of esterase isozymes in radish and B. juncea hybrid F1 (RSjF1-1) by the vertical plate polyacrylamide gel electrophoresis was compared with the zymogram of esterase isozymes in the same period From the mother of the enzyme band, but also from the father of the enzyme band, is complementary to the parents. In addition there are two parents do not have the enzyme band. The comparison of the enzyme pattern of the flower bud esterase isozyme of RSjF1-1 with the pattern of the esterase isoenzyme of the same period showed that the result was consistent with the result of zymogram analysis of the leaves and was still the complement of the parents and had 2 Enzyme band that parent does not have. In the esterase isozyme zymogram of RSjF1-1, there are several bands which are not found in the parents of the two parents, and their activity is strong or strong. The result of multiple repetition shows that they are new bands. Prove RSjF1-1 is a new type of genetic. Indeed for the radish and mustard intergeneric hybrids.