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目的研究Fas/FasL抗原在Behcet病患者外周血T淋巴细胞的表达及其可能的意义。方法采用流式细胞仪(flow cytometry)及三色荧光双标记细胞免疫荧光技术对26例Behcet病患者和43例正常人外周血T淋巴细胞表面Fas和FasL抗原的表达进行检测。结果 Behcet病CD4+T细胞Fas表达阳性率为25.70%±7.32%，对照组为14.02%±6.30%，二者有显著性差异(P＜0.01)；但CD4+T细胞的FasL表达在2组间无显著性差异。CD8+T细胞Fas表达阳性率为9.47%±6.97%，对照组为3.47%±2.75%，二者有显著性差异(P＜0.01)，但FasL表达阳性率二者无显著性差异。结论 Behcet病患者外周血CD4+和CD8+T细胞Fas的表达阳性率高于正常对照组，FasL的表达阳性率相对不足，提示这些活化的Fas+T淋巴细胞不能有效地通过Fas/FasL系统介导的凋亡途径被清除，此可能是Behcet病患者体内大量活化的淋巴细胞长期存在和炎症持续存在及复发的重要原因之一。“,”Objective To evaluate the expression of Fas/FasL antigen on peripheral blood T lymphocytes and its possible role in Behcet disease. Methods The expression of Fas and FasL antigen on peripheral blood T lymphocytes was determined by flow cytometry and three-colour double marked immunofluorescence methods in 26 patients with Behcet disease and 43 healthy individuals. Results The difference was significant between Behcet disease (25.70%±7.32%) and controls (14.02%±6.30%) concerning the Fas expression on CD4+ T lymphocytes(P＜0.01) . But no difference was found concerning the expression of FasL antigen between Behcet disease and controls (P＞0.05).The expression of Fas antigen on CD8+ T lymphocytes from Behcet disease (9.47%±6.97%)was significantly higher than that in control group(3.47%±2.75%), but no difference was found concerning FasL antigen expression on CD8+ T lymphocytes between Behcet disease and controls(P＞0.05).Conclusion These results indicate the imbalance of the expression of Fas and FasL on T lymphocytes in Behcet disease is responsible for the perpetuation and recurrence of Behcet disease for the activated lymphocytes would not be eliminated through apoptosis mediated by Fas/FasL system.