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[目的]观察川芎嗪和大黄酸2种单体联用(简称芎黄联用)治疗猪血清诱导的大鼠肝纤维化的效果,并探讨其可能作用机制。[方法]将48只雄性SD大鼠随机分为5组:正常对照组、模型对照组、大黄酸组、川芎嗪组及芎黄联用组,各治疗组在造模同时分别用相应药物干预,于16周末处死全部大鼠。分光光度比色法检测肝组织匀浆丙二醛(MDA)、超氧化物歧化酶(SOD);免疫组织化学方法观察Smad 4、Smad 7、转化生长因子β1(TGF-β1)、结缔组织生长因子(CTGF)的表达及其在细胞中的定位。[结果]与模型对照组比较,芎黄联用组的肝组织中MDA水平明显降低,SOD水平明显升高;大鼠肝组织内Smad4、TGF-β1、CTGF表达明显降低,Smad 7表达明显升高,肝纤维化程度明显减轻。[结论]芎黄联用能有效地减轻猪血清诱导的肝纤维化大鼠的肝损伤及纤维化程度,其机制可能与干预肝内脂质过氧化反应及抑制TGF-β1和CTGF表达有关。
[Objective] To observe the effect of tetramethylpyrazine and rhein used in the treatment of liver fibrosis induced by porcine serum, and to explore its possible mechanism. [Methods] 48 male SD rats were randomly divided into five groups: normal control group, model control group, rhein group, ligustrazine group, and huanghuanglian group. Each treatment group was treated with corresponding drugs during modeling. All rats were sacrificed at the end of the 16th week. Spectrophotometric colorimetric assay was used to detect malondialdehyde (MDA) and superoxide dismutase (SOD) in liver homogenate; immunohistochemistry was used to observe Smad 4, Smad 7, transforming growth factor β1 (TGF-β1) and connective tissue growth. Expression of factor (CTGF) and its localization in cells. [Results]Compared with the model control group, the level of MDA in the liver tissue was significantly decreased and the level of SOD was significantly increased in the combined group of Qi-Huang-Huang group; the expression of Smad4, TGF-β1, and CTGF in the liver tissue of the rats was significantly reduced, and the expression of Smad 7 was significantly increased. High, liver fibrosis significantly reduced. [Conclusion] Combined use of Radix Paeoniae Alba can effectively reduce liver injury and fibrosis in pig serum-induced hepatic fibrosis. The mechanism may be related to the intervention of intrahepatic lipid peroxidation and the inhibition of TGF-β1 and CTGF expression.