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目的了解甘肃省白银市食源性疾病病人中致泻大肠埃希氏菌(diarrheogenic Escherichia coli,DEC)的分布、毒力基因和耐药性,为制定有效的治疗措施提供参考依据。方法 2016年从白银市347份食源性疾病病人粪便标本中分离并用多重PCR方法鉴定DEC,再结合生化实验验证,根据临床实验室标准化协会(CLSI)推荐的微量肉汤稀释法进行药敏试验。结果 347份粪便标本中共检出DEC 123株,检出率为35.45%;其中肠聚集性大肠杆菌(enteroaggregative Escherichia coli,EAEC)检出最多(74株,21.33%),肠产毒性大肠杆菌(enterotoxigenic Escherichia coli,ETEC)和肠致病性大肠杆菌(enteropathogenic Escherichia coli,EPEC)各23株(各为6.63%),肠侵袭性大肠杆菌(enteroinvasive Escherichia coli,EIEC)3株(0.86%),未检出肠出血性大肠杆菌(enterohemorrhagic Escherichia coli,EHEC);347例病人中,男性200例,检出DEC 66株、阳性率为33.00%,女性147例,检出DEC 57株、阳性率38.78%;5岁及以下儿童196例,检出DEC 75株、阳性率38.27%,19岁~共104例,检出DEC 37株、阳性率35.58%;123株DEC对氨苄西林、四环素和复方磺胺耐药性较高。结论白银市食源性疾病病人中DEC感染普遍存在,并以EAEC、ETEC和EPEC菌株感染为主,菌株的耐药性较强、耐药谱广,基因型呈多态分布。
Objective To understand the distribution, virulence genes and drug resistance of diarrheogenic Escherichia coli (DEC) in patients with food-borne diseases in Baiyin, Gansu Province, and provide a reference for the development of effective therapeutic measures. METHODS: A total of 347 food-borne disease patient stool specimens from Baiyin City were isolated and identified by multiplex PCR in 2016. Combined with biochemical experiments, susceptibility testing was performed according to the CLSI recommended microdilution method . Results A total of 347 123 strains of faecal samples were detected, with a detection rate of 35.45%. Among them, enteroaggregative Escherichia coli (EAEC) was the most detected (74, 21.33%), enterotoxigenic 23 strains of Escherichia coli (ETEC) and enteropathogenic Escherichia coli (EPEC) (23 strains each, 6.63%), 3 strains (0.86%) of enteroinvasive Escherichia coli (EIEC) Enterohemorrhagic Escherichia coli (EHEC) was detected in 347 patients. Of the 200 males, DEC 66 strains were detected, with a positive rate of 33.00% and 147 females, of which 57 were found. The positive rate was 38.78% A total of 196 children aged 5 years and younger were enrolled in this study, and 75 DECs were detected, the positive rate was 38.27% and the age was 19 to 104. DEC 37 strains were detected with a positive rate of 35.58%. 123 DECs were resistant to ampicillin, tetracycline and sulfamethoxazole Sex is higher. CONCLUSION: The prevalence of DEC infection in food-borne diseases in Baiyin City is predominant. Infection with EAEC, ETEC and EPEC strains is dominant. The strains are highly drug-resistant, broad-spectrum resistant and polymorphic.