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目的探讨葛根总黄酮(PR)对人早幼粒细胞白血病(APL)NB4细胞株的影响。方法MTT法检测NB4细胞增殖抑制率;FITC-AnnexinⅤ/PI双染法检测细胞凋亡率;Western blot检测c-Jun氨基端激酶(JNK)、Bcl-2、PARP、半胱天冬酶(Caspase)3蛋白表达的变化。结果PR呈时间-剂量依赖性抑制NB4细胞增殖,诱导细胞凋亡;JNK、PARP及Caspase3的表达与PR浓度呈正相关,而与凋亡抑制蛋白Bcl-2呈负相关。结论PR可有效诱导NB4细胞凋亡,其作用与JNK信号途径的活化有关。
Objective To investigate the effect of Pueraria total flavone (PR) on human promyelocytic leukemia (APL) NB4 cell line. Methods The inhibitory rate of proliferation of NB4 cells was detected by MTT assay. The apoptosis rate of NB4 cells was detected by FITC-AnnexinⅤ / PI double staining. The expressions of c-Jun N-terminal kinase (JNK), Bcl-2, PARP and caspase ) 3 protein expression changes. Results PR inhibited the proliferation of NB4 cells in a time-and dose-dependent manner and induced apoptosis. The expression of JNK, PARP and Caspase3 was positively correlated with the concentration of PR and negatively correlated with the apoptosis-inhibitory protein Bcl-2. Conclusion PR can effectively induce the apoptosis of NB4 cells, and its role is related to the activation of JNK signaling pathway.