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目的观察淫羊藿甙对去卵巢大鼠骨组织Cbfal表达的影响。方法 54只雌性SD大鼠随机分成6组9只/组:假手术组、模型组、尼尔雌醇组、淫羊藿甙低剂量组、淫羊藿甙中剂量组和淫羊藿甙高剂量组。对尼尔雌醇组和淫羊藿甙低、中、高剂量组分别给予尼尔雌醇(0.1mg/kg)和淫羊藿甙(40,80和160mg/kg)治疗12周。12周后,以DXA法测定大鼠全身的骨密度;放射免疫法测定各组大鼠血清中骨钙素及雌二醇的浓度;处死各组大鼠,直接从头盖骨中提取总RNA,采用荧光定量PCR技术检测Cbfal mRNA的相对表达量。结果 12周后,模型组大鼠的骨密度和血清雌二醇水平明显降低,与假手术组相比,差异有显著性(P<0.05)。与模型组相比高剂量组的全身骨密度和血清骨钙素含量增加(P<0.05),但是血清雌二醇的水平无变化(P>0.05)。12周后,与假手术组相比,模型组大鼠骨组织中Cbfal mRNA表达明显降低,差异有显著性(P<0.01)。与模型组相比高剂量组骨组织中Cbfal mRNA的表达,显著升高(P<0.01)。结论淫羊藿甙对去卵巢大鼠骨质疏松症具有防治作用,其部分机制可能为淫羊藿甙促进骨组织中Cbfal表达,从而调节骨形成。
Objective To observe the effect of Epimedium on Cbfal expression in bone tissue of ovariectomized rats. Methods Fifty-four female Sprague-Dawley rats were randomly divided into 6 groups of 9/group: sham operation group, model group, nilestriol group, epimedium low-dose group, epimedium middle-dose group and epimedium Dosage group. Nilestriol (0.1 mg/kg) and Epimedium (40, 80, and 160 mg/kg) were administered to the nilestriol group and Epimedium low, middle, and high dose groups, respectively, for 12 weeks. After 12 weeks, the bone density of rats was determined by DXA method; the serum osteocalcin and estradiol concentrations were determined by radioimmunoassay; the rats were sacrificed and total RNA was directly extracted from the skulls. Real-time PCR was used to detect the relative expression of Cbfal mRNA. Results After 12 weeks, bone mineral density and serum estradiol levels in the model group were significantly lower than those in the sham group (P<0.05). Compared with the model group, the whole body bone density and serum osteocalcin content in the high dose group increased (P<0.05), but serum estradiol level did not change (P>0.05). After 12 weeks, compared with the sham group, the expression of Cbfal mRNA in the bone tissue of the model group was significantly lower (P<0.01). The expression of Cbfal mRNA in the high-dose group was significantly higher than that in the model group (P<0.01). Conclusion Epimedium has preventive and therapeutic effects on osteoporosis in ovariectomized rats. Part of its mechanism may be that epimedium promotes the expression of Cbfal in bone tissue and regulates bone formation.