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脑缺血性损伤是严重危害人类健康的常见病、多发病,可发病于各年龄段,如脑卒中、外伤、中毒、感染、变性等均可导致神经元不可逆性功能丧失。该病具有发病、致残和死亡率高的特点,因而脑缺血损伤性疾病的实验研究越来越受到临床及有关基础医学的重视。理想的全脑缺血损伤动物模型是实验研究能否取得顺利进展的决定性因素之一,而蛛网膜下腔用药是目前临床镇痛及神经保护类药物的常用施药途径。我们采用Pulsinelli四血管阻断全脑缺血损伤模型结合枕骨大孔处蛛网膜下腔置管术构建一重复性高、死亡率低的大鼠全脑缺血损伤模型。由于以上脑缺血模型和蛛网膜下腔置管术均存在手术难度大、大鼠死亡率高等特点,我们对该模型进行了改进,并应用于虎纹蜘蛛毒素(HWTX I)的神经保护作用研究,经5 0多例大鼠动物实验证明,这是一种能产生严重脑缺血,置管定位准确稳定可靠的大鼠全脑缺血损伤模型。
Cerebral ischemic injury is a common disease that seriously endangers human health. It can occur frequently in all age groups, such as stroke, trauma, poisoning, infection, degeneration, etc., and can lead to the irreversible loss of neurons. The disease has the characteristics of morbidity, disability and high mortality. Therefore, the experimental research of cerebral ischemic injury has been paid more and more attention in clinic and related basic medicine. The ideal animal model of global cerebral ischemia is one of the decisive factors for the successful progress of experimental research. However, subarachnoid administration is the common route of clinical analgesia and neuroprotective drugs. We used Pulsinelli four-vessel occlusion model of global cerebral ischemia combined with suboccipital foramen magnum in the construction of a repetitive high-mortality rat model of global cerebral ischemia. Due to the difficulty of operation and the high mortality of rats in the above cerebral ischemia model and subarachnoid catheterization, we improved the model and applied it to the neuroprotection of HWTX I Study, more than 50 rats animal experiments show that this is a can produce severe cerebral ischemia, catheter positioning accurate and reliable rat global cerebral ischemia model.