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目的:研究白屈菜红碱(chelerythrine,CHE)对宫颈癌细胞(Hela)的增殖抑制作用,为CHE在预防和治疗宫颈癌方面提供实验依据。方法:不同浓度CHE作用于体外培养的Hela细胞后,采用台盼蓝拒染法和噻唑蓝(MTT)法研究白屈菜红碱对宫颈癌(Hela)细胞增殖能力的影响,流式细胞仪(FCM)检测细胞周期变化。结果:细胞接种密度越小、药物作用时间越长、药物浓度越大, CHE对Hela细胞的增殖抑制率越大,过大的细胞接种密度以及过低的药物浓度均不会引起明显的细胞死亡;CHE作用后的Hela细胞周期分布有明显变化,G1期细胞减少,S期和G2/M期细胞明显增多。结论:CHE在体外能明显抑制Hela细胞生长,其机理可能与CHE阻滞细胞周期、诱导细胞凋亡有关。
Objective: To study the inhibitory effect of chelerythrine (CHE) on the proliferation of cervical cancer cells (Hela) and to provide experimental evidence for the prevention and treatment of cervical cancer. Methods: The effects of different concentrations of CHE on Hela cells cultured in vitro were studied by trypan blue exclusion assay and MTT assay. The effects of chelerythrine on the proliferation of Hela cells were studied by flow cytometry (FCM) to detect cell cycle changes. Results: The smaller the cell inoculation density, the longer the drug effect, the greater the drug concentration, the greater the inhibition rate of CHE on Hela cells proliferation, too large cell seeding density and too low drug concentration did not cause significant cell death The cell cycle distribution of Hela cells after CHE treatment significantly changed. The cells in G1 phase decreased and the cells in S phase and G2 / M phase increased significantly. Conclusion: CHE can significantly inhibit the growth of Hela cells in vitro. The mechanism may be related to CHE blocking cell cycle and inducing cell apoptosis.