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目的分析研究乙型肝炎病毒(HBV)脱氧核糖核酸(DNA)与血清标志物(HBV-M)的关系。方法 HBV感染患者血清中HBV-DNA含量采用实时荧光定量聚合酶链式反应(PCR)检测,HBV血清标志物采用酶联免疫吸附法测定。结果乙型肝炎e抗原(Hbe Ag)(+)标本HBV-DNA阳性率为100.0%,明显高于(Hbe Ag)(-),比较差异有统计学意义(P<0.05)。结论患者的HBV-DNA阳性率与HBV-M之间存在相关性,采用实时荧光定量聚合酶链反应法检测HBV-DNA,可清晰准确显示患者体内病毒复制情况,之后为临床预防治疗提供可参考的依据。
Objective To analyze the relationship between hepatitis B virus (HBV) DNA and serum markers (HBV-M). Methods The serum HBV-DNA levels in patients with HBV infection were detected by real-time fluorescence quantitative polymerase chain reaction (PCR) and the serum HBV markers were determined by enzyme-linked immunosorbent assay. Results The positive rate of HBV-DNA in Hbe Ag (+) specimens was 100.0%, which was significantly higher than that in Hbe Ag specimens (P <0.05). Conclusion The positive rate of HBV-DNA in patients with HBV-M is related to the use of real-time fluorescence quantitative polymerase chain reaction detection of HBV-DNA, can be clearly and accurately show the patient’s virus replication, and then provide a reference for clinical prevention and treatment The basis.