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In this paper the mixing of a sample in the curved microchannel with heterogeneous surface potentials is analysed numerically by using the control-volume-based finite difference method. The rigorous models for describing the wall potential and extal potential are solved to get the distribution of wall potential and extal potential, then momentum equation is solved to get the fully developed flow field. Finally the mass transport equation is solved to get the concentration field. The results show that the curved microchannel has an optimized capability of sample mixing and transport when the heterogeneous surface is located at the left conjunction between the curved part and straight part. The variation of heterogeneous surface potential ψn has more influence on the capability of sample mixing than on that of sample transport. The ratio of the curved microchannel’s radius to width has a comparable effect on the capability of sample mixing and transport. The conclusions above are helpful to the optimization of the design of microfluidic devices for the improvement of the efficiency of sample mixing.