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目的探讨MMP2及MMP9在膀胱移行细胞癌患者尿液中的表达,并研究它们与膀胱移行细胞癌侵袭转移之间的关系。方法采用酶联免疫吸附法(ELISA)定量检测尿液中MMP2及MMP9的含量,并分析其表达水平与膀胱移行细胞癌临床分期和病理分级之间的关系。结果MMP2在膀胱移行细胞癌患者尿液中的含量明显高于非癌症患者,二者差异有统计学意义(P<0.01);MMP9在膀胱移行细胞癌患者尿液中的含量也明显高于非癌症患者(P<0.01)。MMP9在膀胱移行细胞癌患者尿液中的含量与肿瘤临床分期呈显著正相关(r=0.51361,P<0.01);与肿瘤病理分级也呈显著正相关(r=0.47378,P<0.01)。MMP2在膀胱移行细胞癌者尿液中的含量与肿瘤临床分期呈正相关(r=0.32271,P<0.01);而与肿瘤病理分级不相关(r=0.29818,P>0.05)。MMP2和MMP9在膀胱移行细胞癌患者尿液中的含量呈显著正相关(r=0.55674,P<0.01)。结论膀胱移行细胞癌患者尿液中增高的MMP2及MMP9与肿瘤细胞侵袭能力密切相关。进一步研究对指导临床治疗、判断预后有重要意义。
Objective To investigate the expression of MMP2 and MMP9 in urinary bladder transitional cell carcinoma (BTCC) and their relationship with invasion and metastasis of bladder transitional cell carcinoma (BTCC). Methods The levels of MMP2 and MMP9 in urine were quantitatively determined by enzyme-linked immunosorbent assay (ELISA), and the relationship between the expression of MMP2 and MMP9 in bladder transitional cell carcinoma and clinical stage and pathological grade were analyzed. Results MMP2 in urinary bladder transitional cell carcinoma patients was significantly higher than non-cancerous patients, the difference was statistically significant (P <0.01); MMP9 in bladder transitional cell carcinoma in patients with urine was significantly higher than non- Cancer patients (P <0.01). The level of MMP9 in urinary bladder transitional cell carcinoma was positively correlated with clinical stage (r = 0.51361, P <0.01), and positively correlated with tumor grade (r = 0.47378, P <0.01). The expression of MMP2 in urinary bladder transitional cell carcinoma was positively correlated with clinical stage (r = 0.32271, P <0.01), but not with tumor pathological grade (r = 0.29818, P> 0.05). There was a significant positive correlation between MMP2 and MMP9 in urine of bladder transitional cell carcinoma (r = 0.55674, P <0.01). Conclusion The increased urinary MMP2 and MMP9 in bladder transitional cell carcinoma are closely related to the invasiveness of tumor cells. Further research is of great importance to guiding clinical treatment and prognosis.