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目的探讨CD3+CD16+56+NK T细胞百分率在慢性乙型肝炎(CHB)患者外周血中的变化及其与肝损伤的相关性。方法用流式细胞术检测29例中重度CHB患者、26例轻度CHB患者及30例健康对照者外周血CD3+CD16+56+NK T细胞的百分率;同时用全自动生化分析仪测定此三组对象血清ALT的水平。结果中重度CHB组外周血CD3+CD16+56+NK T细胞百分率(3.55%±1.45%)显著低于轻度CHB组(4.86%±1.97%,P<0.05)及健康对照组(5.84%±2.08%,P<0.01),轻度CHB组与健康对照组差异无统计学意义(P>0.05);中重度CHB组外周血CD3+CD16+56+NK T细胞百分率与血清ALT水平呈显著负相关(r=-0.501,P<0.01),轻度CHB组外周血CD3+CD16+56+NK T细胞百分率与血清ALT水平无显著相关性(r=-0.199,P>0.05)。结论外周血CD3+CD16+56+NK T细胞百分率在CHB慢性进展的各个临床阶段可能有所不同,且其变化趋势与肝损伤的程度呈负相关。
Objective To investigate the changes of the percentage of CD3 + CD16 + 56 + NK T cells in peripheral blood of patients with chronic hepatitis B (CHB) and its correlation with liver injury. Methods The percentage of CD3 + CD16 + 56 + NK T cells in 29 CHB patients, 26 mild CHB patients and 30 healthy controls were detected by flow cytometry. The level of serum ALT in the target group. Results The percentage of CD3 + CD16 + 56 + NK T cells in peripheral blood of moderate and severe CHB group was significantly lower than that of mild CHB group (4.86% ± 1.97%, P <0.05) and healthy control group (5.84% ± 2.08%, P <0.01). There was no significant difference between mild CHB group and healthy control group (P> 0.05). The percentage of CD3 + CD16 + 56 + NK T cells and serum ALT level in CHB group were significantly negative (R = -0.501, P <0.01). The percentage of CD3 + CD16 + 56 + NK T cells in peripheral blood of mild CHB group was not significantly correlated with serum ALT level (r = -0.199, P> 0.05). Conclusion The percentage of CD3 + CD16 + 56 + NK T cells in peripheral blood may be different at different clinical stages of chronic CHB progression, and the change trend is negatively correlated with the degree of liver injury.