【摘 要】
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The strain LWYZ003,which can restrain multiple pathogens,was screened from the sediment of the ocean and identified as Bacillus amyloliquefaciens.Large-scale fe
【机 构】
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School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China“,”Key Labor
论文部分内容阅读
The strain LWYZ003,which can restrain multiple pathogens,was screened from the sediment of the ocean and identified as Bacillus amyloliquefaciens.Large-scale fermentation and mod chromatographic separation technologies (macroporous resin column chromatography,silica gel column chromatography,thin-layer chromatography and high-performance liquid chromatography) were used to separate antimicrobial products from the fermentation broth of marine-derived Bacillus amyloliquefaciens LWYZ003.Bioactive-guided separation was used in the term of seeking antimicrobial products from the secondary metabolites of Bacillus amyloliquefaciens LWYZ003.As a result,two natural products cycloheximide (1) and trehalose (2) were obtained.Their structures were elucidated by Fourier transform infrared spectroscopy,high-resolution mass spectrometry,1H and 13C nuclear magnetic resonance analysis.In the cylinder plate method,compound 1 exhibited stronger antimicrobial activities than compound 2 against Micrococcus luteus,and also exhibited wider antimicrobial spectrum than compound 2.In conclusion,isolation of bioactive secondary metabolites from marine Bacillus sp.has enormous potentials in finding suitable antibiotics to inhibit multiple pathogens.
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