作者应用电泳荧光微量测定法测定了肌萎缩侧索硬化症(ALS)患者,酒精中毒者和对照者共三组人的血浆及脑脊液的游离疏胺和硫胺单磷酸盐含量。分组时注意排除营养不良及血脑屏障损害对测定结果的影响。结果表明ALS组的血浆及酒精中毒组的血浆和脑脊液中总硫胺(硫胺+硫胺单磷酸盐)浓度均较对照组明显下降,而硫胺/硫胺单磷酸盐比率(T/TMP)则不变。ALS组的脑脊液中总硫胺浓度较对照组明显下降,其中硫胺单磷酸盐减少更明显,故T/TMP的比值增大显著。一般认为硫胺单磷酸盐是由于硫 The authors used electrophoretic fluorimetry to determine free sparse amines and thiamine monophosphates in plasma and cerebrospinal fluid in patients with amyotrophic lateral sclerosis (ALS), alcoholism and controls. Care should be taken when grouping to exclude the effects of malnutrition and blood-brain barrier damage on the test results. The results showed that plasma and cerebrospinal fluid concentrations of total thiamin (thiamin + thiamine monophosphate) in the ALS group were significantly lower than those in the control group, whereas the ratio of thiamine to thiamine monophosphate (T / TMP ) Does not change. ALS group cerebrospinal fluid total thiamine concentration decreased significantly compared with the control group, in which the reduction of thiamine monophosphate more obvious, so the T / TMP ratio increased significantly. Thiamin monophosphate is generally believed to be due to sulfur