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本文探讨双价志贺疫苗滴鼻免疫小鼠一段时间后,粘膜免疫和系统免疫应答的变化。将BALB/c小鼠随机分为三组,每组30只。PBS、FSM-2117和FS-5416(菌量为5×10~6、1×10~7、4×10~7和4×10~7CFU/只/次)经滴鼻途径免疫小鼠。间隔2周,4次免疫后7、30和90d活杀,收集鼻咽、肺、肠、生殖道冲洗液和血清。采用ELISA法检测其中特异性抗福氏、宋内LPSIgA和IgG。结果是两株疫苗经鼻内免疫后,诱发鼻咽、肺、胃肠道和生殖道等不同粘膜部位及血清中特异性抗福氏、宋内LPSIgA、IgG的显著增加(P<0.01)。特异性抗体水平虽然在免疫后30、90d明显下降,但仍明显高于PBS对照组水平。故认为两株双价志贺疫苗滴鼻免疫小鼠后能有效诱导粘膜免疫和系统免疫应答,并持续较长时间。
This article investigates the changes of mucosal immunity and systemic immune response after mice immunized with bivalent Shiga vaccine intranasally for a period of time. BALB / c mice were randomly divided into three groups of 30. PBS, FSM-2117 and FS-5416 (5 × 10 ~ 6, 1 × 10 ~ 7, 4 × 10 ~ 7 and 4 × 10 ~ 7CFU / After 2 weeks of immunization, the mice were killed at 7, 30 and 90 days after 4 immunizations. Nasopharynx, lung, intestine and reproductive tract rinse and serum were collected. The specific anti-Forsythia, LPSIgA and IgG were detected by ELISA. The results showed that after intranasal immunization, the two vaccines induced a significant increase (P <0.01) of LPSIgA and IgG in the different mucosal parts of the nasopharynx, lung, gastrointestinal tract and genital tract, and in the serum specific anti-Forsythia and Song. Although the level of specific antibody decreased significantly at 30 and 90 days after immunization, it was still significantly higher than that of PBS control group. Therefore, two bivalent Shiga vaccine intranasally immunized mice can effectively induce mucosal immunity and systemic immune response, and continued for a long time.