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目的 分析N 乙酰化转移酶 1 (NAT1 )各等位基因型及其与散发性大肠腺癌 (SCRAC)易感性之间的关联。方法 应用聚合酶链反应 (PCR) 限制性片段长度多态性分析 (RFLP)及多重PCR技术 ,检测中国湖北地区汉族无血缘关系的健康人 1 0 1例、SCRAC患者 1 0 4例的NAT1基因多态性。结果 (1 )NAT1等位基因 1 0 (NATI 1 0 )频率在SCRAC患者与健康人之间及老年SCRAC患者与健康人之间的差异均有显著性 (前者P <0 0 5 ,OR =1 91 ,95 %CI=1 1～ 3 4;后者P <0 0 1 ,OR =2 91 ,95 %CI =1 48～ 5 90 ) ;在近端或远端SCRAC与健康人之间的差异无显著性 (P >0 0 5) ;(2 )DukesⅡ期、Ⅲ和Ⅳ期分别与健康人相比 ,其NAT1 1 0的频率差异均有显著性 (前者P <0 0 5 ,OR =2 48,95 %CI=1 1 8～ 5 2 0 ;后者P <0 0 5 ,OR =3 0 4 ,95 %CI =1 2 8～ 7 2 0 )。结论 NAT1 1 0与SCRAC易感性有关 ,其患者肿瘤浸润、转移早 ,与癌灶部位无关 ;是否与老年SCRAC易感性有关尚不清楚
Objective To analyze the association of each allele of N-acetyltransferase 1 (NAT1) and its susceptibility to sporadic colorectal adenocarcinoma (SCRAC). Methods The polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) and multiplex PCR were used to detect the association of unrelated healthy Han nationality in China with 101 unrelated healthy subjects and 104 NATR genes in SCRAC patients Polymorphism. Results (1) There was a significant difference in the frequency of NAT1 allele 10 (NATI 10) between SCRAC patients and healthy subjects and between elderly SCRAC patients and healthy subjects (P <0.05, OR = 1 91, 95% CI = 1 1 to 34; the latter P <0 0 1, OR = 2 91, 95% CI = 1 48 to 5 90); differences between proximal and distal SCRACs and healthy subjects (2) The frequencies of NAT1 1 0 in Dukes Ⅱ, Ⅲ and Ⅳ were significantly different from those in healthy people (P <0.05, OR = 2 48, 95% CI = 118 ~ 520; the latter P <0 05, OR = 340, 95% CI = 128 ~ 720). Conclusion NAT1 1 0 is associated with the susceptibility to SCRAC. The invasion and metastasis of the tumor are not associated with the tumor site. It is unclear whether NAT1 1 0 is associated with SCRAC susceptibility.