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为研究番茄miRNA在非生物逆境胁迫下的表达模式和功能分析。利用Real-time PCR检测番茄miRNA397在非生物逆境(干旱、盐害、ABA)条件下的表达量变化,发现Sly-miR397响应这些逆境胁迫,尤其在干旱胁迫下表达最明显。故将Sly-miR397过表达载体转入拟南芥中,进行转基因功能验证。结果表明:与野生型相比,转基因拟南芥植株叶片相对含水量下降速率更缓慢,保水能力更好,且在干旱胁迫下,转基因植株的长势明显优于野生型,其最大光合效率、3种抗氧化酶活性SOD、POD、CAT均明显高于野生型,同时胁迫所产生的丙二醛含量明显低于野生型拟南芥。表明Sly-miR397能提高拟南芥对干旱胁迫的耐受性,在植物抗旱过程中起着重要作用。
To study the expression pattern and function of tomato miRNA under abiotic stress conditions. Real-time PCR detection of tomato miRNA397 under abiotic stress (drought, salt damage, ABA) expression changes found that Sly-miR397 response to these stress conditions, especially under drought stress expression of the most obvious. Therefore, Sly-miR397 overexpression vector was transformed into Arabidopsis thaliana for transgenic function verification. The results showed that compared with the wild type, the relative water content of transgenic Arabidopsis leaves decreased more slowly and had better water retention capacity. Under drought stress, the growth of transgenic plants was significantly better than that of wild type. The maximum photosynthetic efficiency The antioxidant enzyme activities of SOD, POD and CAT were significantly higher than those of wild type, and the content of malondialdehyde produced by stress was significantly lower than that of wild type Arabidopsis thaliana. This indicated that Sly-miR397 could enhance the tolerance of Arabidopsis thaliana to drought stress and play an important role in plant drought resistance.